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Reconfigurable T-junction DNA origami

Katherine G. Young, Behnam Najafi, William M. Sant, Sonia Contera, Ard A. Louis, Jonathan P. K. Doye, Andrew J. Turberfield and Jonathan Bath

Angew. Chem. Int. Ed. 15942-15946 (2020)

ToC entry


DNA self-assembly allows construction of nanometre-scale structures and devices. Structures with thousands of unique components are routinely assembled in good yield. Experimental progress has been rapid, based largely on empirical design rules. Here we demonstrate a new DNA origami technique designed as a model system with which to explore the mechanism of assembly. The origami fold is controlled through single-stranded loops embedded in a double-stranded DNA template and is programmed by a set of double-stranded linkers that specify pairwise interactions between loop sequences. Assembly is via T-junctions formed by hybridization of single-stranded overhangs on the linkers with the loops. The sequence of loops on the template and the set of interaction rules embodied in the linkers can be reconfigured with ease. We show that a set of just two interaction rules can be used to assemble simple T-junction origami motifs and that assembly can be performed at room temperature.

The full paper is available from Angewandte Chemie.